FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49796485
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG133950.011.43905738
CGGTTTA84250.011.18999638
GGCATAG137450.010.9829235
TTCGTCG88200.010.8445453
AGCTTAG141900.010.79752845
GTTTCGT93950.010.7317281
TTTCGTC92800.010.5495112
GGGAGGT221700.010.3950193
GGAGGTC226900.010.1368824
CGGCTTA110600.010.11009430
GTCACTC221700.09.9660258
GTTGTCG25400.09.83232726
TAGCACG24100.09.61533729
CATAGGG164300.09.27118437
TCGTCGA106250.09.17163854
TGGCATA167100.09.12863634
ACTCTCC248650.08.948297511
CGTCGAG112550.08.9377325
GCATAGG172900.08.79659136
TCACTCT265400.08.6634759
Produced by FastQC (version 0.11.5)