FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48004560
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT51100.011.4887271
TTCGTCG46900.010.8364363
CGGTTTA48600.010.47321138
TAGCACG31750.010.015305529
TCGCGCT29150.09.88261545
TTTCGTC53800.09.8234312
ATAGGGG74050.09.12439238
GGCATAG75800.08.89570335
CGGCTAA79950.08.74443231
CATTCGG49250.08.5833044
CGGCTTA57700.08.48150230
CATAGGG81400.08.46591637
GCATTCG47600.08.2676493
GCTCGCG33000.08.26561343
TTAACGG54500.08.1669488
CGTGGCG87500.07.9690391
CTCGCGC34300.07.8080844
GCATAGG88200.07.720577236
ATCGGTT72650.07.67170436
CGAGTTA40000.07.667738434
Produced by FastQC (version 0.11.5)