FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49268133
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG22800.011.74438926
ACCCGGC58400.011.3258155
GGCATAG82100.011.07278735
ATAGGGG88100.010.625360538
CGGCTAA105800.010.54897731
CATAGGG92400.010.00893137
GCATAGG94450.09.79167236
AGCTTAG98450.09.45969845
CGGACAC68350.09.38145913
TGTCGTT31100.09.18890728
TAGCACG19900.09.04605529
ATAGCTC68550.09.02724139
AGGGATC144100.08.9141022
TTACGCG17200.08.76526635
GGTCACT148550.08.5568837
CGCTGAT155650.08.5564721
GGGAGGT177450.08.4814843
CCGGACA78050.08.44622712
GTCACTC154300.08.39839658
ATATAGC38650.08.3820066
Produced by FastQC (version 0.11.5)