FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50801873
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG23350.017.14927721
GTCCGTT35450.016.8179041
TCGACGA74950.016.49379345
CCCCCCT41100.015.600741
TAAACCG80100.015.58506511
CTCCGGT59100.015.03667451
ACGCGTC8100.015.00735340
CTCGACG83600.014.94838144
ACCGCGT39200.014.000556
ATGCGCG13350.013.66229445
CTCCGAT55750.013.5996341
CCCTAAC30750.013.5353281
AACCGAT93650.013.52224313
GTCCGGT38000.013.4987471
GACCGCG40350.013.4900355
CCTTACG54900.013.20358141
CCGCTAT29750.013.1584421
ATCGAGC53400.013.11292642
AGACCGC41750.013.0376764
CCCCGTT58850.012.9979441
Produced by FastQC (version 0.11.5)