FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49477749
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG16400.017.42267621
GTCCGTT32000.016.9443071
TCGACGA61750.015.46352845
CCCCCCT37500.014.9991111
CTCCGGT58550.014.9478751
CCCGTAT38050.014.6640441
TAAACCG63450.014.57027511
CTCGACG67900.014.22849244
GCACTAC73550.014.19461825
CCCAGAT199550.014.0482781
AGACGCT217300.013.93125141
GCTCGGC193400.013.91525445
GTCCGAT27500.013.8264541
AAGCGGC210650.013.58734728
CCTTACG49100.013.43116541
GTCGAAA47850.013.4004581
CTCGGTT80750.013.2623711
CCCGGAT90700.013.2461831
CTCCGAT54050.013.028831
CCGCAGA238150.013.02116337
Produced by FastQC (version 0.11.5)