FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51049750
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA848000.16611246871923957No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT828870.16236514380579728No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCTC573930.11242562402362401No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAAA563350.11035313591153727No Hit
CTCCTTCTGTGCGCAGTTTGTAGTAGTTCTTACACTGGTAGCGAACCGAGT558210.10934627495727206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG168200.024.7096795
AGACCGC171250.024.4141354
ACCGCGT173550.023.9868566
CAGACCG179250.023.4626143
CCGCGTT184550.022.7002937
GAACGAT191000.022.1222545
CGCGTTC190550.021.9500878
TCAGACC207900.020.8462542
TCGACGA203450.020.23766945
ATCGAGC117500.019.6269642
CTCGACG215600.019.24328644
GCGTTCT224050.018.7084949
AGAACGA230450.018.41331944
TAAACCG230300.018.02495411
CCCAAAT275400.018.015251
AATACGG234750.017.8948132
TCTCGAC232750.017.7383643
ATACGGA240600.017.53452533
AGATCGA97350.017.33394815
TACGGAG243050.017.2559434
Produced by FastQC (version 0.11.5)