FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51049750
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGTT516980.1012698397151798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT135500.014.2234921
TTTCGTC134000.014.068282
CGGCTTA182000.013.93500230
TTCGTCG134050.013.7778523
TAGCGTA4100.013.17598337
CGGTTTA141800.012.85765838
CAGGGGT204500.012.44861840
CGTCGAG156650.012.2363875
TCGTCGA157850.012.099694
TCGGCTT211750.012.0728429
GTCGAGA162200.011.7893636
AGGGGTG227150.011.50986741
GTTGTCG24350.011.45929826
GGTGTGT230700.011.39812244
ATCGGTT162300.011.31689536
AGCTCGG230250.011.15119326
GCTCGGC227150.011.11564427
TTAGCTC232950.011.06529924
TCGGTTT167350.011.01574337
GGCATAG75400.010.89625435
Produced by FastQC (version 0.11.5)