FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48112596
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG830530.17262215491344512No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG603180.12536841703573842No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT583470.12127177672973623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG45500.026.1982486
CGACGAG48600.023.3239738
GTTCGAC55300.021.6368665
GACCGCG105950.020.039135
ACCGCGT107150.019.9196576
AACGCTA193150.019.74345823
AGACCGC111450.019.635524
ACGCTAC195300.019.57226824
CGGTATA72250.019.55253820
GCCGGCT186650.019.1915171
TCACGCT207200.018.9523358
CGCTACC202000.018.9231125
TTCACGC211800.018.5725577
CAGACCG123000.017.9014453
AAAACGC214450.017.81243521
CTCGACG123800.017.7619844
CCGGCTT229150.017.746162
CCGCGTT119900.017.7263977
CGCGTTC120800.017.5943478
GAAAACG220650.017.351520
Produced by FastQC (version 0.11.5)