FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48112596
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31300.013.66943426
TTCGTCG79800.013.0924973
GTTTCGT91900.012.9656731
CGGCTTA112150.012.39896930
TCAGACG124200.012.355422
TGTCGTT38100.012.18529528
CGTCGAG89500.012.126715
TTTCGTC91150.012.1027272
TCGTCGA90100.011.9205124
CGGTTTA88400.011.85844138
ATATAGC44600.011.4376
TGCCCGT180150.011.23877444
GTCGAGA103750.010.6777556
AACTAAC314950.010.47114937
TCGGCTT135150.010.35546929
ACTAACC310250.010.33272238
AACTCTA114600.010.1236945
GGCATAG119650.010.046926535
CAGACGT152350.09.999073
GCCCGTG204500.09.95573945
Produced by FastQC (version 0.11.5)