FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46914068
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTTA76050.011.98215730
ATAGGGG113400.011.21086538
GGCATAG110700.010.58912535
CGTAACG15550.010.56337542
GGAGGTC158550.010.2913284
GTTTCGT69400.010.05314351
CACCGAA230950.010.0439345
GGGAGGT167950.09.9699413
TAATCGG76800.09.81714
TCTATAG230600.09.7890663
TTCTATA248150.09.7769662
GCACCGA223250.09.72562144
TTCGTCG56150.09.6597733
CGGCTAA94500.09.64292731
CATAGGG129550.09.53520137
TAGCACG24100.09.52258629
CGCGTTC12150.09.44399914
AATCGGT80800.09.41457755
GTAACGA77850.09.392590518
CTATAGT241100.09.3534144
Produced by FastQC (version 0.11.5)