FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49596070
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG507380.1023024606586772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG36500.016.77472126
GTCACTC267050.014.0336288
GGTCACT274250.013.6816037
TGTCGTT45050.013.49331928
ATATAGC50900.013.4794116
GGGAGGT300600.013.470193
GGAGGTC292450.013.30713754
ATAGGGG192650.013.1388538
TATAGCT55900.013.0785357
TTGTCGT48500.012.90476927
GGCATAG195500.012.87847935
GCTAAGT49750.012.70566214
AGGTCAC308100.012.6384616
ACTCTCC303450.012.45396111
TCACTCT319150.012.1725869
GAGGTCA327650.011.8912855
CACTCTC325000.011.773519510
TGGCATA220300.011.49105734
AGCTTAG224900.011.36294445
GTCGTTC55800.011.17623129
Produced by FastQC (version 0.11.5)