FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46056254
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC27900.017.445062
TCGACGA64900.017.1258645
CGTTACG20600.016.9244821
CTCCGGT58550.016.876081
GTCCGTT34400.016.8538481
TAAACCG71250.015.69025411
CCCGTAT28000.014.9052091
CTCGACG74250.014.8454244
TCGCGTG3200.014.7874152
ACCGCGT33200.014.7021246
CCGCAAT41750.014.5892051
GACCGCG34050.014.3603665
CCCCCCT34900.014.3499991
AACCGAT82050.013.87206713
CTCGCGT8650.013.8224211
GCACTAC58100.013.627892525
CCGGCTT117400.013.60821152
TTCACGC101300.013.5227897
TCGTTAC11500.013.49721344
CCCGGAT73650.013.4773421
Produced by FastQC (version 0.11.5)