FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49993645
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1237410.2475134589606339No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG712250.14246810769648824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG39100.029.0616446
CGACGAG49850.022.8380378
GTTCGAC50000.022.593125
CGGTATA64900.021.63276520
GACCGCG298750.021.625585
AGACCGC307650.021.3803084
ACCGCGT303100.021.3802176
GAACGAT311850.021.1899945
CAGACCG318050.020.8651453
CGCGTTC314850.020.6093858
CCGCGTT315550.020.5577477
TCAGACC360850.019.1885412
CCGGCTT263800.018.9885392
TCACGCT259200.018.9405778
TTCACGC261000.018.8455457
AACGCTA240850.018.39382623
GCGTTCT353750.018.3748829
ACGCTAC242050.018.32122624
GCCGGCT243350.018.2927381
AAAACGC241450.018.04060621
Produced by FastQC (version 0.11.5)