FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47578194
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1639360.34456120801895085No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT994390.2090012075700057No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGTC631120.1326490030285723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT403900.026.2515831
CCGGCTT489350.024.1075842
TTCGACG84750.023.6023146
TTCACGC510950.023.3249057
TCACGCT522200.022.7879628
CGGCTTC538250.022.4860063
GACCGCG244000.021.9933345
ACCGCGT246250.021.929446
ACGCTCA554200.021.72795310
AGACCGC251300.021.7216644
CACGCTC552100.021.6353879
GTTCGAC94600.021.5253375
CGACGAG93050.021.0605938
AACGCTA518300.021.05367723
ACGCTAC518850.020.9793224
CAGACCG264500.020.8247833
CCGCGTT259700.020.7578897
CGCTACC526000.020.64281725
CGCGTTC264550.020.4028688
AAAACGC531350.020.37656821
Produced by FastQC (version 0.11.5)