FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47578194
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG741920.15593698239155526No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA547400.11505270670845555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA160950.013.41583845
AGGGATC198650.012.3556272
TATATAC88050.012.2320857
CAGGGAT231500.011.73339751
ATATATA103750.010.6583666
GGAGGTC192950.010.6219014
CATGCAT94950.010.3262399
GGGAGGT211650.010.0341913
ATAGGGG151500.010.00334138
ATATACA117400.09.9211278
TCCATAT124550.09.8984113
GGCATAG150950.09.86065835
AGCTTAG154000.09.75648245
GGGATCA257750.09.7320613
TTCCATA134750.09.5665312
GTTGTCG22500.09.49839126
AACTAAC125850.09.48342637
CCATATA110800.09.4821274
ACGACTG103550.09.4380336
TACATTC137450.09.31082511
Produced by FastQC (version 0.11.5)