FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49608247
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG792940.1598403588016323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT215100.021.9374072
TTCGACG19150.020.9147916
TTCACGC213800.020.8273837
TCACGCT222200.020.1301528
GCCGGCT207950.019.9777241
AACGCTA223700.019.60289223
ACGCTAC224400.019.49161124
ACGCTCA235250.019.04214910
CGCTACC230700.018.93982525
CACGCTC239450.018.5860129
TAAACCG126750.018.35468311
AGACCGC58750.018.0018734
AAAACGC244800.017.95921921
GAAAACG246400.017.80607620
CGGCTTC300550.017.1004313
AAACGCT259100.017.06355322
GCGGACT78400.017.0202482
GTTCGAC24200.017.0162335
TCGACGA137150.016.9464745
ATCGAGC98350.016.88327642
Produced by FastQC (version 0.11.5)