FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005481996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005481996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50216325
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT5665561.1282307098339037No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1489590.29663461035828487No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1185350.23604873514738484No Hit
GTGCAAATTAAATTCAGAAGGGACGGGGGAAACAGAGTCGTGGAGGCTTTG627570.1249733030045508No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC558990.11131638964022159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT796600.034.14896445
AGAACGA981400.027.85630644
AGACCGC1067550.025.8623074
GACCGCG1067450.025.8479465
ACCGCGT1068350.025.7860536
CAGACCG1073500.025.7775973
CGCGTTC1075650.025.6110538
CCGCGTT1079050.025.5303577
TCAGACC1108850.025.1022552
GCGTTCT1120650.024.6228149
AAGAACG1113650.024.49819443
CTCAGAC1176700.024.3345581
AATACGG1141800.023.86287332
ATACGGA1150700.023.66657433
TACGGAG1149050.023.63990634
CGTTCTC1176500.023.5612210
CAATACG1175400.023.21518531
CCCAATA1187050.023.12191829
ACGGAGA1179550.023.05364435
CACTCCC1208950.022.83751724
Produced by FastQC (version 0.11.5)