FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42093333
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA89950.018.986245
GAACGAT83550.018.15143245
CTCGACG96000.017.78956844
TAAACCG100300.017.29526311
CGTTACG21000.016.82112921
ACCGCGT93950.015.925746
CTCCGGT48900.015.87809851
AACCGAT110700.015.71106413
GACCGCG95000.015.6313925
AGACCGC96050.015.5777674
GTCCGTT29750.015.3566791
AAACCGA114400.015.24226112
CCCCCCT31950.015.2149721
ATCGAGC51950.014.94271242
CTCCGAT47050.014.8282641
CAGACCG101850.014.8244483
TCTCGAC117250.014.69965643
CCCGTAT27150.014.672081
TCGGCGC33500.014.57512845
CTCCGTT77150.014.3229951
Produced by FastQC (version 0.11.5)