FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42093333
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG33750.012.53227829
GTTGTCG19650.010.076097526
CGAGTTA44150.09.83519234
CGGCTTA82600.09.66954730
CGGTTTA61900.09.48721338
CGGCTAA85900.09.455235531
ACTAGTA48100.09.12160124
CACACGC90650.09.061794
ACACGCC92100.08.9675375
TTTCGTC69950.08.9443582
ACTTAGG77800.08.79190138
TTCGTCG67800.08.7632723
GTTTCGT76800.08.6739911
CGTAAAC16100.08.666711
CTAGTAG53500.08.6634525
CGCGTAT6850.08.542231
CACGAGT51500.08.4315832
TCGTCGA73100.08.4049334
TACTAGT50700.08.299252523
TAGGCCA87450.08.28499241
Produced by FastQC (version 0.11.5)