FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50090447
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG779520.15562248825609404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA208650.021.68234323
ACGCTAC208050.021.58267624
CGCTACC213750.020.70188325
TCACGCT236200.020.2390968
AAAACGC226250.020.08533921
TTCACGC244850.019.4873437
GAAAACG235800.019.34823820
CCGGCTT252250.019.3004192
GCCGGCT240950.018.5062431
AAACGCT248100.018.38897722
ACCGCGT72800.018.2319326
ACGCTCA267200.018.15199510
AGACCGC73900.018.1438874
GACCGCG74350.017.912255
CACGCTC273000.017.7581089
CGGCTTC302800.016.702023
GTTTTCG273750.016.6212145
CGCGTTC79900.016.6118228
CGTGGTT283350.016.30979241
CAGACCG85900.016.2116053
Produced by FastQC (version 0.11.5)