FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50090447
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG174100.013.38874145
GGCATAG187650.012.361423535
ATAGGGG190350.012.174252538
GGGAGGT271300.011.9998993
GGAGGTC277800.011.6867274
GGTCACT278900.011.2050747
TGGCATA212550.011.040318534
GTCACTC294200.010.9206228
GAGCTTA221350.010.70333144
ACTCTCC315250.010.25618111
GTTTCGT42050.010.1659151
AGGTCAC329450.09.9842686
GAGGTCA340350.09.7306285
TCACTCT351150.09.3739789
CATAGGG254050.09.22799537
CACTCTC360250.09.02476910
GTTGTCG26200.08.93220126
GCATAGG262400.08.88289936
TCAGACG108600.08.7840512
TTCGTCG39400.08.7368343
Produced by FastQC (version 0.11.5)