FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47224658
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG175050.012.84159538
GGCATAG179500.012.54761835
AGCTTAG183250.012.46181945
GGAGGTC265200.012.4296924
GGGAGGT268950.012.4237053
GTCACTC269500.011.919858
GGTCACT277400.011.6945547
TGGCATA198000.011.35236634
GAGCTTA201800.011.23969344
TAGCACG43900.011.17404129
AGGTCAC301850.011.0304926
ACTCTCC304600.010.70148411
CATAGGG218350.010.36649137
CGGCTTA74700.010.33188130
GAGGTCA325400.010.2821665
GTTGTCG22950.010.197626
CGAGTTA47100.010.0319334
GCATAGG226250.010.0241936
TCACTCT328950.010.0113119
CACTCTC323550.09.99813210
Produced by FastQC (version 0.11.5)