FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51986385
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG54650.024.910436
CGGTATA79000.022.2147920
CGACGAG63700.021.1218438
GTTCGAC69750.019.7111995
GACCGCG121200.018.9742365
ACCGCGT125000.018.523436
AGACCGC127750.018.3360544
AACGACG38900.018.04610316
GAACGAT134500.017.89920245
CAGACCG137100.017.4794673
TCGACGA81300.016.9091117
CCGCGTT138200.016.8012567
AGTTCGA85100.016.4466294
CGCGTTC143750.016.1369298
ACGAGGA107100.015.83988210
CGAGGAT97950.015.73475511
CGGTATG65750.015.33035921
GTATAGA124150.015.31369722
ATGACGG109400.015.28093416
CAGTTCG97400.015.2706853
Produced by FastQC (version 0.11.5)