FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46950937
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1077580.2295119264605944No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA688420.14662540174650826No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCTC578700.12325632606650641No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTTT501670.10684983773593273No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAAA483290.10293511288177273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT189800.027.88158845
AGAACGA255350.020.77707344
TCGACGA162650.020.22412345
ATCGAGC120850.019.6790842
CTCGACG179750.018.5755444
TCGGCGC81700.018.28615645
GACCGCG290000.018.1395175
AGACCGC291000.018.0931644
ACCGCGT294550.017.8592536
CGAGCTT136200.017.7420444
CAGACCG301500.017.5010973
AAGAACG307150.017.28040143
CCGCGTT306250.017.1695567
CGCGTTC309100.016.9966878
TCGAGCT142150.016.90444243
TAAACCG197500.016.7921711
ATCTCGA201650.016.5023742
TTCGGCG91900.016.50140244
TCAGACC327650.016.4353962
TCTCGAC202450.016.28156943
Produced by FastQC (version 0.11.5)