FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47619227
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT626760.1316191041908345No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA513920.10792279345483706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG156350.020.3167955
ACCGCGT157450.020.1175866
AGACCGC158000.020.062114
CAGACCG167000.019.1561723
TCGGCGC58900.018.44859145
CCGCGTT172200.018.3813257
CTCGACG141700.017.9401444
CGCGTTC177550.017.8655018
TCGACGA143150.017.77471445
ATCGAGC101200.016.85023342
TTCGACG20800.016.7642546
GACGAAA63150.016.6726245
TAAACCG152100.016.63975511
AACCGAT155700.016.24057213
CCCGTAT29750.016.1308731
CCCAAAT215650.015.995251
TCAGACC203350.015.9311712
TTCGGCG69800.015.72829544
GAACGAT196500.015.46764445
AAACCGA167800.015.08287512
Produced by FastQC (version 0.11.5)