FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47619227
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG98700.012.91734138
GGCATAG103700.012.27206635
AGCTTAG108350.011.82839245
CATAGGG110450.011.74426937
GTTTCGT92650.010.9086511
GCATAGG116300.010.88273236
GTCACTC169000.010.56261358
TTCGTCG88350.010.3841833
TGGCATA126350.010.285048534
TAGCACG26200.010.05745429
CGGTTTA94200.09.85190438
CGTCGAG98200.09.8226785
GGTCACT178450.09.81428057
TTTCGTC98600.09.761842
GAGCTTA135950.09.57615144
TCGTCGA99800.09.5302424
GTTGTCG21950.09.524631526
ACCCGGC57100.09.411225
GGAGGTC202050.09.3256544
GGGAGGT213550.09.2767343
Produced by FastQC (version 0.11.5)