FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46298237
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC643340.13895561509177984No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG562630.1215229858536514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT33950.016.4116781
CCCCCCT34800.014.45511151
GTCCCGT42000.014.0717411
CTCCGTT65500.014.0168021
CCCGTAT39100.013.9615761
AGACGCT169700.013.93175441
CTCGGTT86300.013.9058231
ATGTCGC179650.013.72341535
CCCCGCA164250.013.70905535
AAGCGGC163400.013.6697828
CCTTACG60000.013.64693441
CCGCAGA177050.013.63288637
CGCGCTA1000.00742985613.5346521
TGTCGCT185500.013.39976636
CTCCGAT47400.013.2776641
CCCCGTT52100.013.0324061
GCAGACG179750.013.02764239
GTGGGAC268650.013.02049737
GCTCGGC165000.013.00708245
CAGACGC186800.012.89727640
Produced by FastQC (version 0.11.5)