FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45694670
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA991580.21700123887534367No Hit
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGTT563910.12340826621573152No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC551670.12072961682401907No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG497460.10886608875827312No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG494200.10815265762943468No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG468720.10257651494145817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG25600.018.09956726
ATATAGC30800.017.3915186
AGGGATC154650.015.7172472
CGTGGCG247550.015.6801531
GGGAGGT248950.015.2512563
CGTCGAT39250.015.13683312
GTCACTC244700.015.1215628
GTGGCGA256750.015.0158382
TGTCGTT31800.014.92330228
GCGTCGA38050.014.90432611
GGAGGTC252450.014.7811094
CAGGGAT178900.014.67861
TATAGCT34950.014.6186587
TGCGTCG39550.014.56693210
CAGACGT136000.014.1465751
AGGTCAC266300.014.1226616
GCTAAGT35200.013.93947614
TGGCGAC281600.013.7786683
GTCGTTC35200.013.54560829
GGGATCA180700.013.4389683
Produced by FastQC (version 0.11.5)