FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48802879
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTCTGTGCGCAGTTTGTAGTAGTTCTTACACTGGTAGCGAACCGAGT559620.11466946447974923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC30250.020.23286432
ACGTTCG21000.020.0350046
TCGACGA111100.018.7929845
CTCGACG114450.018.32230644
GTCCGTT37650.018.2315831
TCGGCGC44450.018.01938845
TAAACCG117400.017.86102911
ACCGCGA13450.017.73149545
ACCGCGT41900.017.2905566
TCGTATG36450.017.15967445
ATCGAGC61950.016.85270542
TGCGCGT27000.016.6658745
AGACCGC43300.016.6275864
CTCCGGT53050.016.502651
AACCGAT127550.016.47498713
ATGCGCG27850.016.07710644
CCCGTAT22350.015.9099581
CGTCCCG40950.015.66072735
ACAACGG55650.015.56641142
TCACGCT126550.015.3073378
Produced by FastQC (version 0.11.5)