FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48802879
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC92050.019.4560555
TCACCCG99800.018.3716093
CGGACAC100050.017.58638413
CGGACAG107450.016.35505519
CCGGACA108400.016.33549512
CACCCGG116200.016.0118584
GCCCGGA114200.015.62417310
ACGGACA115500.015.56544218
CACGGAC115550.015.11089617
CGGCCCG123200.014.6274948
GACACGG123750.014.32797415
GCGTCGT44300.013.76438711
GGTCGCC43100.013.5185143
CCCGGAC134950.013.48860411
ACACGGA150700.012.15410316
ATAGCTC141550.012.01706239
TCTCGGT53700.011.98392636
TAGCACG48100.011.88228529
CCCGGCC157150.011.7395796
CGGCTTA94900.011.35679430
Produced by FastQC (version 0.11.5)