FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47442224
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1192040.251261407981211No Hit
GGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACA821490.17315587903298968No Hit
GTGCAAATAAAATTCAGAAGGGACGGGGGAAACAGAGTCGTGGAGGCTTTG780490.1645137883923823No Hit
GGGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGAC765540.16136258704903886No Hit
GGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACAG595410.12550212654448914No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA549570.11583984764289297No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG509890.10747599016437341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT274500.024.3189951
TTCGACG28100.023.4563226
TCACGCT415000.023.325018
TTCACGC417450.023.2527837
CCGGCTT327000.022.9024072
CACGCTC452650.021.384919
ACGCTCA467850.021.03633310
GACCGCG105750.020.759035
CGGCTTC377150.020.555573
ACCGCGT105900.020.5201156
AGACCGC108550.020.1623974
AACGCTA473050.020.11699323
ACGCTAC474900.020.04374524
CGCTACC483050.019.65019625
AAAACGC490150.019.4746721
GAAAACG500450.019.0378920
AAACGCT504250.018.9479622
CTTCACG527800.018.7449596
CAGACCG118050.018.7115463
GTTCGAC36350.018.2106635
Produced by FastQC (version 0.11.5)