FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45255824
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG80400.017.434565
ACCGCGT81700.017.0745076
AGACCGC83500.016.7609184
GAACGAT86400.016.66642245
CTCCGGT61750.016.5110471
GTCCGTT38200.016.4971161
CCGCGTT89150.015.6475897
CGCGTTC91900.015.20383558
CCCGTAT28450.014.951781
TAAACCG58250.014.87102511
TCGACGA58500.014.73055245
TTCGACG14850.014.54532056
CAGACCG98550.014.5213453
CCCCCCT43650.014.2826541
CTCCGAT49300.014.2436421
CTCGCGT8000.014.0667381
CCGTACG41000.013.939394
TCGGCGC40950.013.68111745
TTCGGCG42850.013.59956644
CGTACGC42200.013.5958625
Produced by FastQC (version 0.11.5)