FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45255824
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG67350.010.85755938
GTTGTCG23150.010.6903226
GGCATAG75750.09.50437535
CGGCTTA49850.08.981730
CATAGGG80000.08.9716237
AGCTTAG81650.08.76295845
ATATAGC36200.08.7024486
GCATAGG81700.08.67473536
GTTTCGT41400.08.4252581
TGTCGTT29200.08.32163528
CGGTTTA36900.08.17080638
GTCACTC130250.08.0844018
TTCGTCG38000.07.8763353
TAGCACG17450.07.865061829
TCAGACG60000.07.83875042
GGAGGTC151800.07.50127174
CGTAACG9600.07.50031742
GGGAGGT145300.07.49610853
GCTAAGT34850.07.36008414
ACTTAGG69200.07.315818338
Produced by FastQC (version 0.11.5)