FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52133085
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1596180.30617409270907336No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA715040.13715666356594855No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCTC560430.10749987268161859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC296450.027.1838074
GACCGCG295300.027.152865
ACCGCGT297000.027.0125856
GAACGAT297700.026.95984545
CAGACCG304850.026.4934353
CGCGTTC315050.025.5078748
CCGCGTT315050.025.4792447
TCAGACC337800.024.5287422
GCGTTCT345750.023.3080189
AGAACGA355650.022.59306544
CTCAGAC414900.021.3908081
CGTTCTC390700.021.12151110
AATACGG379650.021.1086632
TACGGAG381000.020.95738234
ATACGGA382700.020.94036933
ATCGAGC123950.020.54116842
CAATACG394150.020.4236331
AAGAACG401100.020.04651543
ACGGAGA408650.019.66652535
GATCGAA104450.019.4871916
Produced by FastQC (version 0.11.5)