FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45399942
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG17200.016.613036
GTCCGTT35150.016.0712151
CCCGTAT30650.016.0076051
CCCCCCT31950.015.919811
CTCCGGT58200.015.4294431
ACGTCCG20300.015.40618832
CTCCGAT48650.015.1274331
CTCCGTT75200.014.3356711
CCCGGAT91650.014.2428151
TCACGCT120350.014.1713298
CCCCCGT25300.013.8772821
AGACCGC48800.013.4166744
CTCGCGT8550.013.1614651
ACCGCGT49650.013.1417236
AACGCTA121250.013.11944823
CGATATA43250.012.90145421
ACGCTAC122200.012.8885724
CTACACG73700.012.85254445
ACGTTCG12500.012.7797376
CCCCCTT99900.012.7512071
Produced by FastQC (version 0.11.5)