FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45399942
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG19900.010.62952226
TAGCACG21650.09.873529
ACCCGGC57850.09.801495
CGCCGTA12150.09.62898145
TCACCCG68850.09.0853273
GGCATAG68100.08.45825235
GCGTCGT37750.08.40256411
ATAGGGG76950.08.33388438
CGGACAC63400.08.16131613
TAATCGG32300.08.0808144
GGTCGCC42350.08.02291843
CGGACAG65550.07.894439719
TCAGACG66700.07.8938662
CACCCGG76200.07.85464144
GTAACGA29300.07.83240118
ACGGACA75150.07.814007818
TTAGGCC91000.07.813719740
CGGCCCG74800.07.78942638
ACTTAGG93550.07.696927538
ATAATCG39400.07.65258743
Produced by FastQC (version 0.11.5)