FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43786143
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT2696810.6159048994107564No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTT1376550.31438028236467414No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT1271900.2904800269802252No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG1170490.26731973172425805No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG998830.2281155478800679No Hit
CCCTCGTCCAACCAGCTGCCACGTCCTGTAACCAAAAGTGACCGGGATGAA995500.2273550333035728No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG951200.21723767722587484No Hit
GGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACA930190.21243935552852874No Hit
GGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACAG700990.1600940279211165No Hit
GGGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGAC659540.15062756269717567No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC654940.14957700202093618No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTT617420.141008081026913No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAAT565500.12915044835074876No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCC541140.12358704442179344No Hit
GCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT518790.11848268983180364No Hit
GGCGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGG516220.11789574614964374No Hit
GGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA510550.11660081592480068No Hit
GTTAAATCTTCCCCGCAGACCGACTCGACCAGTGAGCTATTACGCTTTCTT488280.11151473195526722No Hit
CGGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA484820.11072452762053053No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC461830.10547400806689002No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCT461440.10538493879216537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT217700.025.4821171
TTCGACG41450.025.0701716
TTCACGC309700.024.9904127
TCACGCT314650.024.4757188
CCGGCTT251650.023.928782
CGACGAG43800.023.3136988
ACGCTCA338050.022.97447410
GTTCGAC47500.022.9662695
CACGCTC335250.022.9248059
CGGCTTC277800.022.2580853
CGGTATA59700.021.9271720
AACGCTA352600.021.73340223
ACGCTAC352700.021.67622224
AAAACGC368800.020.8579521
GAAAACG368750.020.68986920
TTAGTTT691750.020.59856645
GTTAGTT696700.020.29037544
AAACGCT380350.020.21866822
CGCTCAG381950.020.14540511
AGTTCGA56550.019.6090554
Produced by FastQC (version 0.11.5)