FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43786143
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC1946810.44461783263257515No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT1154070.2635696868755944No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC850780.19430348089805488No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT757420.1729816668255069No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA732100.16719901545107546No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAA696970.15917592924318544No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG638380.14579498358647391No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAA632150.1443721590184365No Hit
GCCGTCCCCACCACCTCCCTCTTCTTCTTTTTCATCCTTCTGTCTCTTTGT630900.1440866805738062No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA587850.13425480294073858No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTA524570.11980274215977414No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT465660.10634871402123726No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC458750.10477058917932094No Hit
ATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCA446740.10202771228331302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG301150.017.324572
GGTAGGC88250.016.69338831
GAACGCT98350.016.6248621
AACGCTG100350.016.24056822
GCCTAAC89600.016.1462536
ATCAGAC338050.015.3995311
TGCCCGT210000.015.09391144
GTAGGCC95700.014.88468132
GGCCTAA98350.014.79407835
AGGATTC993350.014.702167545
CGGTAGG102550.014.03681430
TAGGCCT105350.013.66807633
ACTAACC1027450.013.50030438
GCCCGTG236050.013.49230245
CTAACCA1033150.013.44980839
CCAGGAT1041950.013.34287543
AACTAAC1094500.013.31280437
CTAACAC111600.013.25125538
GGCGGTA108350.013.244596528
CAGACGT408050.013.1600751
Produced by FastQC (version 0.11.5)