FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47945480
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG592450.12356743534531305No Hit
CCTCTGTCTTGTTGGGGGTGAAGTAAAAGAAGGTTGCTTGGATCTCCTGAA541700.11298249595165175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG21450.022.5440356
CGACGAG25350.018.8982338
GACCGCG60100.018.8615155
AGACCGC60700.018.8234464
ACCGCGT59750.018.8214286
TAAACCG105550.017.87819911
CGTATAA54750.017.8704913
CTCGTAT76750.017.8211731
ACGCGTC13400.017.81851640
GTTCGAC27500.017.4207735
CAGACCG70400.016.5176893
GTCCGTT33400.016.2323591
CGATATA62350.015.90857321
AACCGAT121800.015.65934513
CACGCGT16350.015.56698139
TCGACGA121600.015.35595745
CCCGTAT29600.015.3522041
CCCAAAT195300.015.2048721
CCCATAT151550.015.1410261
TCGTATA73550.015.0151672
Produced by FastQC (version 0.11.5)