FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49104754
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1434970.292226288314162No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG537220.10940284926384114No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT516460.10517515269499161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC266100.032.0558784
CAGACCG271850.031.410963
GACCGCG278650.030.5792055
ACCGCGT279850.030.4557196
CCGCGTT277850.028.9824167
CGCGTTC284350.028.2959358
TCAGACC310150.027.8948062
GAACGAT309400.027.13939745
AATACGG318800.026.69205932
TACGGAG321500.026.36291934
ATACGGA322550.026.34685533
CTCAGAC370900.025.8691221
GCGTTCT316950.025.406799
AGAACGA334250.025.1553644
ACGGAGA345000.024.5867535
CCCAATA355150.023.97276929
CAATACG369600.023.04160731
CCCCAAT380650.022.45547928
CGGAGAG377900.022.4343236
CGTTCTC369750.022.00387210
Produced by FastQC (version 0.11.5)