FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41323027
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG517810.12530785801340255No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG511630.12381232381645227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA174050.020.98055623
ACGCTAC176400.020.68779824
CGCTACC181250.020.13431725
TCACGCT189000.019.90378
TTCACGC189600.019.7339157
CCGGCTT195250.019.7169082
GCCGGCT172300.019.6324961
ACGCTCA191100.019.53211410
AAAACGC194750.018.93526521
GAAAACG192350.018.92588220
CACGCTC203450.018.3685539
AAACGCT203100.018.0571922
TGTACTA66850.017.60207413
CGTTACG22200.017.43192521
TACTATC70350.017.23805415
GACCGCG75400.017.188125
AGTGTAC70800.016.87426411
ACCGCGT77050.016.8196956
GTACTAT72550.016.3431914
CGTGGTT203950.016.20703941
Produced by FastQC (version 0.11.5)