FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41323027
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTTA70100.012.4267430
GTTTCGT61350.012.0642571
CGTAACG7150.011.96245342
TGCCCGT125350.011.43846744
TTCGTCG53500.011.0151663
TCAGACG80600.010.6054572
TCGTCGA59350.010.4224994
TTTCGTC58400.010.2844092
CGTGGCG136050.010.1694131
CGGTTTA62400.09.6304238
CGTCGAG66700.09.5100575
TAATCGG49100.09.4372534
GCCCGTG152550.09.41440145
GTGGCGA145300.09.3818372
TGGCGAC149250.09.2533213
TCGGCTT94500.09.24195229
GCGACCC150450.09.2097625
CGACCCG151000.08.9671776
ATAGGGG67900.08.85034138
AGCTCGG101250.08.84403426
Produced by FastQC (version 0.11.5)