FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49822733
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1179680.23677544947203116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT210450.026.69118345
AGACCGC250150.022.265474
ACCGCGT249600.022.1613226
GACCGCG252150.021.9550485
CAGACCG257600.021.7350333
AGAACGA261400.021.54198544
CCGCGTT259450.021.3292397
CGCGTTC266450.020.7688488
AATACGG286550.019.4861632
ATACGGA293000.019.07299433
TACGGAG293700.019.01263434
GCGTTCT295250.018.8191939
TCAGACC307550.018.7975312
GTCCGTT37950.018.259071
AAGAACG313200.017.93030243
CAATACG319900.017.58132231
ACGGAGA324450.017.25982735
ACGTTCG20700.017.1699286
CTCAGAC369500.016.8900811
TCGACGA75850.016.63515745
Produced by FastQC (version 0.11.5)