FastQCFastQC Report
Wed 11 May 2022
EGAF00005482670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49681165
Sequences flagged as poor quality0
Sequence length0-98
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGC3084180.6207946210601141No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGG2493410.501882353201661No Hit
AGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGG2427810.48867815398451303No Hit
GGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGC2047210.41206964450209654No Hit
ATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGG1847070.37178475987831605No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1775470.3573728595132582No Hit
GACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCGCT1723810.34697455262975413No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAG1671070.33635885953962635No Hit
AGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG1573660.3167518314033095No Hit
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGG1430450.28792601783794725No Hit
GGAGCCCGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTC1309940.26366934028217737No Hit
AACGGAGCCCGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGC1260550.2537279469996326No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA1226780.24693060237214645No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCTCGCTTCTGG1204880.2425224931822754No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1048930.21113232751285121No Hit
GGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCGCTCGTTC970990.1954442896015019No Hit
GAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGG935610.18832287849932666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG918400.18485878904007183No Hit
GGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGC829070.16687813178294028No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT794840.15998819673411444No Hit
AGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCC792700.1595574499913599No Hit
AACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCT788850.15878250842145109No Hit
AAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAA710070.1429253923493944No Hit
GCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCGCTCGT664420.13373679944904673No Hit
AAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAA661480.1331450258865709No Hit
GGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCGCTCGTTCGTTCGCCGCCC641310.1290851371943472No Hit
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG588580.1184714569394659No Hit
GTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCGCTCGTTCGTTCG588270.11840905904682388No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGA583040.11735634621289578No Hit
GGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCC544560.10961095618430043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)