FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005482809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38529041
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1276220.3312358592055276No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG1201290.31178819114651724No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG1146160.29747950383711863No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA936300.24301149878088063No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT839230.21781751588366813No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC690160.1791272199066673No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA614400.15946412992734493No Hit
AGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTTCGCTGCA612250.15890610929039214No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA556790.1445117723018333No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC556350.1443975727296197No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG478500.12419203478228279No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA475420.12339263777678765No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA441540.11459927071634096No Hit
GTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTTTGGCGTTGCTTCGCTG406240.10543735049102312No Hit
CGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG389050.10097578084022386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)