FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005482834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42835716
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT5429871.2676034176713658No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA3455160.8066072713713948No Hit
TGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG2540880.5931685605535343No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1931050.4508037171597645No Hit
GCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT1824810.4260019839518966No Hit
TCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG1264070.2950972034645108No Hit
TCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG1106810.25838484875565054No Hit
GGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAA942800.22009670621590638No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG883550.20626479081148077No Hit
GACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGA867600.2025412625296143No Hit
TCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG795330.18566982748695038No Hit
AGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA681170.1590191698908453No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA539600.12596964645110637No Hit
TTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC458140.1069528054579501No Hit
GCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCT447030.10435917541333965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)