FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005482839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47566193
Sequences flagged as poor quality0
Sequence length25-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA2101940.44189788322979723No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1575010.33111962523467037No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG1200860.2524608181276984No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1193230.2508567376834215No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA1091500.22946969920422264No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG1026230.2157477685885015No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA894290.18800958067003595No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA771850.16226860955637126No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT682320.14344641792123242No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG621570.13067474203790075No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA543690.11430176890549135No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA531290.11169487539185656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)