FastQCFastQC Report
Thu 12 May 2022
EGAF00005482840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46904892
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3504610.7471736636767013No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2968150.6328017981578553No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2053660.4378349277512461No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1293150.27569618964264964No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG1070190.22816170219515697No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG884610.18859653274545435No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTG827030.17632062770766No Hit
CAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAG808850.17244469937165616No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA626060.13347435060718188No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT623930.13302024019157746No Hit
CGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTA621050.1324062317423095No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCA617000.13154278236052647No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC509760.10867949552042461No Hit
CTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATC492390.10497625706077737No Hit
CGGCGAAGCAACACCAAAAAGCCAGCTTCACGCTCAGGAGAAAACGCTAC477760.10185717941744755No Hit
CTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATT475800.1014393125561402No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)