FastQCFastQC Report
Thu 12 May 2022
EGAF00005482853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50827188
Sequences flagged as poor quality0
Sequence length25-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1891480.37213941483443863No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA1544680.30390821542202967No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1225850.24117997635438737No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1010740.19885813867963736No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA981470.19309940970962233No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA975530.19193074383733366No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG788810.15519449944781522No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA738970.14538872384598575No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG704730.1386521717471366No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG690490.13585052157518532No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA541460.10652959986690588No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG513830.10109353285489649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)