FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005482854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56426026
Sequences flagged as poor quality0
Sequence length25-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT2551860.45224875485649124No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2296540.40700013146415803No Hit
GGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGGGAG1856200.32896167452940955No Hit
GGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGT1425100.2525607598167555No Hit
GTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGG1380370.24463356678707093No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC1328780.23549062271371016No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1223020.2167474987517285No Hit
TCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCA1168540.2070923796760027No Hit
CACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG1164740.20641893157600713No Hit
CACGCTGCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGG1089770.1931325094558316No Hit
TGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC1084560.19220917666610085No Hit
AGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGG1064530.18865939628638742No Hit
GCTGCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGT867050.1536613618687235No Hit
TTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGG861280.1526387840958355No Hit
GGTCACCACGCTGCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCG803700.14243427314906068No Hit
TGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAG793580.14064077452486198No Hit
CGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCG737290.13066488148571725No Hit
CTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG722790.12809514531468155No Hit
TGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG714530.12663128181311226No Hit
TGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGA703070.12460030412207303No Hit
TGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTG665630.1179650681052747No Hit
AACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCAGA656660.11637537614291675No Hit
TCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA655720.11620878634975994No Hit
AACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGA631020.11183137369978882No Hit
TTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC631000.11182782923610463No Hit
TCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCC623910.11057131686006028No Hit
GGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCC599490.10624352670166777No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG575310.10195827010748551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)